Submission Requirements

Sample Submission Form

  • Genotyping Project

    1. Genomic DNA samples can be submitted in either 1.5mL tubes or 96 well plates
    2. All genomic DNA samples should be at the same concentration with a minimum ratio of 1.7 to 2 by spectrophotometer.
    3. Genomic DNA samples should be submitted at a minimum volume of 50uL.
    4. Genomic DNA must be run on a 1% agarose gel and show no fragments below 2kb.
    5. All genomic DNA must be diluted in 1X TE buffer.
    6. All genomic DNA sample submissions must be accompanied with the sample submission form and all related QC information (gel images and spec readings). If submitting a 96 well plate, ensure that a plate map is included with the submission.
    7. SNP data such as “rs” numbers must be provided in an excel sheet and sent with the sample submission form.
    8. All remaining samples must be collected within 60 days of receiving all results. After 60 days remaining samples will be discarded.
  • Ion Torrent Sequencing Project

    1. Genomic DNA samples must be submitted in 1.5mL LoBind tubes
    2. All samples must be diluted in 0.1X TE buffer
    3. Genomic DNA samples must be run on a 1% agarose gel and show no fragments below 200bp.
    4. Genomic DNA samples must be submitted at a minimum concentration of 35ng/uL by spectrophotometer and in a minimum volume of 100uL. If this is not possible, QGLO also offers sample concentration; see link.
    5. All remaining samples must be collected within 60 days of receiving all results. After 60 days remaining samples will be discarded.
  • DNA and RNA Extraction

    1. Samples being submitted for DNA extraction must be in a sealed container and clearly labelled.
    2. Container labels must not be removable.
    3. All samples must be submitted with any necessary warning labels and the submission form.
    4. All remaining samples must be collected within 60 days of receiving all results. After 60 days remaining samples will be discarded.
  • Ambient DNA and RNA Storage

    1. Samples must be submitted at a minimum concentration of 500ng/uL in a minimum volume of 50uL.
    2. Samples must be submitted in a 1.5mL tube or a 96 well plate.
    3. If aliquots are required there will be an additional fee for each additional aliquot.
    4. All sample submissions must be accompanied with the completed sample submission form.

Sample Submission Instructions

Download the sample submission form and e-mail the completed form to qglo@queensu.ca.

 

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